ABSTRACT
<p><b>OBJECTIVE</b>To research on genetic diversity of different Salvia miltiorrhiza geographical populations in China.</p><p><b>METHOD</b>The genetic diversity of 27 S. miltiorrhiza geographical populations from ten provinces in China was estimated using amplified fragment length polymorphism (AFLP) markers. The data of amplified bands were analyzed by the software POPGENE and SPSS.</p><p><b>RESULT</b>The ten primers employed produced a total of 528 discernable and reproduceable amplified fragments. There were 476 polymorphic brands. The percentage of polymorphic bands with in different populations was 90.15%. Genetic diversity analysis showed that Neis gene diversity (He) was 0.261 2 and Shannon's genetic diversity index (1) was 0.403 3. The coefficient of gene similarity was 0.504 0-0.789 0 between populations. The cluster map including all samples were obtained by UPGMA. In the map, there were seven cluster groups and one individual outside the groups.</p><p><b>CONCLUSION</b>The genetic diversity with in different geographical population of S. miltiorrhiza in China is plentiful.</p>
Subject(s)
Amplified Fragment Length Polymorphism Analysis , China , Cluster Analysis , DNA Primers , DNA, Plant , Genetics , Genetic Variation , Genetics, Population , Phylogeny , Plants, Medicinal , Classification , Genetics , Salvia miltiorrhiza , Classification , GeneticsABSTRACT
<p><b>OBJECTIVE</b>Study on the karyotypes analysis of Ferula fukanensis.</p><p><b>METHOD</b>The young roots were treated with 0.000 2 mol x L(1) 8-Hydroxyquinoline for 3 h, carnoy's for 3 h, 1 mol x L(-1) HCl in 5 min,carbol fuchsin coloration for 2 min and the treated roots were utilizied to make the plate for observation.</p><p><b>RESULT</b>The fukanensis is diploid. The chromosome number of somatic cells was 2n = 22. The karyotype formula is 2n = 2x = 20 = 16m + 4sm. The 4th and 10th are submetacentric, and the others are metacentric.</p><p><b>CONCLUSION</b>The karyotype of F. fukanensis belongs to "2A" type of stebbins', and it is a primitive species.</p>
Subject(s)
Centromere , Chromosomes, Plant , Genetics , Diploidy , Ferula , Genetics , Karyotyping , Plant Roots , Genetics , Plants, Medicinal , Genetics , Seeds , GeneticsABSTRACT
<p><b>OBJECTIVE</b>In order to clarify the genetic background of Pinellia ternata germplasm resources in China, the chromosomal constitution and cytogeographical distribution of P. ternata were investigated in 27 different populations among 16 provinces and regions in China systematically.</p><p><b>METHOD</b>Cytological and cytogeographical methods were used in the study.</p><p><b>RESULT</b>P. ternata in China is a polyploid complex, which contains septuploid (2n = 7x = 91) , octoploid (2n = 8x = 104) , nonuploid (2n = 9x = 117) and decaploid (2n = 10x = 130). Meanwhile the aneuploid series (2n = 92, 103, 105, 115) of a minority of P. ternata were also found.</p><p><b>CONCLUSION</b>The genetic differentiation and the phenomenon of ploidy miscellany commonly exist in the species of P. ternata in China, both for natural populations and cultivated populations. Toxicity and chemical components of different ploidy P. ternata should be clarified before the superior multiploid is selected for normalized plantation of the plant.</p>